After discovering Bunsen's uncle in a museum, Bunsen and Mikey try to sneak him out before Amanda catches them.
- Jeremy Rowley as Bunsen
- Ben Giroux as Mikey Munroe
- Kari Wahlgren as Amanda Killman
- Jeff Bennett as Uncle Funkle
- Cheri Oteri as Miss Flap, Aunt Rhoda
- Chris Hardwick as Officer Steve Stevenson
- It is revealed that that Amanda's dad owned the museum.
- The episode's title is a reference to the 2006 movie "Night at the Museum".
- The title card is similar to "Spelling Beast" and "Tooth or Consequences".
- This is the first episode where Bunsen does not appear in the title card.